Figure 6: Essential gene set conserved between pathogenic streptococci. Venn diagram showing the comparison of the 177 essential genes identified in the GAS core genome to found in (A) Streptococcus For the high-relatedness condition, we used a mutant strain, the hyphae of which lacked the ability to fuse. Each spore of this mutant formed a separate colony with clonally related nuclei, with its Medial pulses flow to and produce steps of shrinkage of the adjacent ‘vertical’ junctions. They subsequently fuse with junctions and sustain junctional Myo-II accumulation, which stabilizes junction The percentages of samples with levels below 0.14 pg/mL (detection limit for unstimulated samples) were: IL-1β 0.08%, IL-1RA 0.03%, IL-6 0.006% and TNF-α 0%. The percentages of samples with levels The full-length AtSHL was cloned into a pET-Sumo vector to fuse a hexahistidine-sumo tag to the target protein. The plasmids were transformed into Escherichia coli strain BL21(DE3) RIL (Stratagene). After normal or pathologic muscle damage, myoblasts proliferate, differentiate, and fuse with each other to form multinucleated myotubes or fuse with myofibers, and reconstitute the contractile .
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